locus

BSU09100

BSU_09100

geneLength

201

204

ec

outlinks

bsu

BSU09100

BSU_09100

Gene

Coordinates

984,262 → 984,465

984,262 984,465

The protein

Paralogous protein(s)

[[protein|CspC]], [[protein|CspD]]

[[this]]

The protein

Structure

[PDB|2ES2] [Pubmed|16780871]

[PDB|1CSP] [Pubmed|8321288]

[PDB|1NMG] (NMR)

[PDB|3PF4] (in complex with r(GUCUUUA)) [Pubmed|22128343]

[PDB|2I5M] [pubmed|17481655]

[PDB|2ES2] [Pubmed|16780871]

[PDB|1CSP] [Pubmed|8321288]

[PDB|1NMG] (NMR)

[PDB|3PF4] (in complex with r(GUCUUUA)) [Pubmed|22128343]

Biological materials

two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in [SW|Stülke] lab

B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

[SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

References

12399512, 12171653, 11752341, 11717297, 12838604, 8321289, 9533624, 9379903, 8294017, 8755892, 9914312, 11591689, 7476164, 1409560, 16352840, 9920884, 1400185, 12427936, 8307174, 7703860, 22128343, 23199363, 15378759, 21124848, 16780871, 26809452, 8321288

12399512, 12171653, 11752341, 11717297, 12838604, 8321289, 9533624, 9379903, 8294017, 8755892, 9914312, 11591689, 7476164, 1409560, 16352840, 9920884, 1400185, 12427936, 8307174, 7703860, 22128343, 23199363, 15378759, 21124848, 16780871, 26809452, 8321288, 17481655, 30738179, 31756296, 32060391, 32786103, 32949815

The protein

[SW|Domains]

[SW|CSD domain] (aa 4-63) (according to UniProt)

Biological materials

Mutant

GP1968 (Δ[[gene|cspB]]::cat trpC2) available in [SW|Jörg Stülke]'s lab

GP3251 (Δ[[gene|cspB]]::tet trpC2) available in [SW|Jörg Stülke]'s lab

BKE09100 (Δ[[gene|cspB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAATTTCCTCCTAAAG, downstream forward: _UP4_TAAGCATAAATTGATATGAA

BKK09100 (Δ[[gene|cspB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAAATTTCCTCCTAAAG, downstream forward: _UP4_TAAGCATAAATTGATATGAA

Biological materials

Expression vector

Constitutive overexpression of Strep-CspB with pGP3125 (in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

Biological materials

lacZ fusion

GP3283 ([[gene|cspB]]::lacZ cat, based on [SW|pAC5]), available in [SW|Jörg Stülke]'s lab

labs

[SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]